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Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone
Ahmad, Fairus; Soelaiman, Ima Nirwana; Ramli, Elvy Suhana Mohd; Hooi, Tan Ming; Suhaimi, Farihah H.
Affiliation
  • Ahmad, Fairus; Universiti Kebangsaan Malaysia. MY
  • Soelaiman, Ima Nirwana; Universiti Kebangsaan Malaysia. MY
  • Ramli, Elvy Suhana Mohd; Universiti Kebangsaan Malaysia. MY
  • Hooi, Tan Ming; Universiti Tunku Abdul Rahman. MY
  • Suhaimi, Farihah H; Universiti Kebangsaan Malaysia. MY
Clinics ; 66(5): 849-853, 2011. ilus, graf
Article in En | LILACS | ID: lil-593851
Responsible library: BR1.1
ABSTRACT

INTRODUCTION:

Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the 11β-hydroxysteroid dehydrogenase type 1 enzyme. Adrenalectomized rats treated with dexamethasone exhibited an increase in visceral fat deposition but not in body weight.

OBJECTIVES:

The main aim of this study was to determine the effect of dexamethasone on the histomorphometric characteristics of perirenal adipocytes of adrenalectomized, dexamethasone-treated rats (ADR+Dexa) and the association of dexamethasone treatment with the expression and activity of 11 β-hydroxysteroid dehydrogenase type 1 (11 β-hydroxysteroid dehydrogenase type 1).

METHODS:

A total of 20 male Sprague Dawley rats were divided into 3 groups a baseline control group (n = 6), a sham-operated group (n = 7) and an adrenalectomized group (n=7). The adrenalectomized group was given intramuscular dexamethasone (ADR+Dexa) 2 weeks post adrenalectomy, and the rats from the sham-operated group were administered intramuscular vehicle (olive oil).

RESULTS:

Treatment with 120 μg/kg intramuscular dexamethasone for 8 weeks resulted in a significant decrease in the diameter of the perirenal adipocytes (p<0.05) and a significant increase in the number of perirenal adipocytes (p<0.05). There was minimal weight gain but pronounced fat deposition in the dexamethasone-treated rats. These changes in the perirenal adipocytes were associated with high expression and dehydrogenase activity of 11β-hydroxysteroid dehydrogenase type 1.

CONCLUSIONS:

In conclusion, dexamethasone increased the deposition of perirenal fat by hyperplasia, which causes increases in the expression and dehydrogenase activity of 11 β-hydroxysteroid dehydrogenase type 1 in adrenalectomized rats.
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Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Dexamethasone / Adipose Tissue / Adipocytes / Intra-Abdominal Fat / Glucocorticoids / Kidney Limits: Animals Language: En Journal: Clinics Journal subject: MEDICINA Year: 2011 Document type: Article / Project document Affiliation country:

Full text: 1 Collection: 01-internacional Database: LILACS Main subject: Dexamethasone / Adipose Tissue / Adipocytes / Intra-Abdominal Fat / Glucocorticoids / Kidney Limits: Animals Language: En Journal: Clinics Journal subject: MEDICINA Year: 2011 Document type: Article / Project document Affiliation country: